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Learn how to make homemade buttermilk with just two simple ingredients! This quick buttermilk substitute is perfect when you’re baking and realize you’re out of buttermilk. In just a few minutes, you’ll have a homemade version that works beautifully in pancakes, biscuits, cakes, muffins, and more.

Once you’ve got your homemade buttermilk, be sure to try my Buttermilk Pound Cake and Buttermilk Pie!

Glass measuring cup with buttermilk.

Why you’ll love this homemade buttermilk recipe!

Making homemade buttermilk couldn’t be easier. It only takes a couple of pantry staples and a few minutes, making it one of the most useful baking tricks to have in your back pocket.

  • Only Two Ingredients: All you need is milk and either lemon juice or white vinegar.
  • Ready in Minutes: The mixture thickens in about 5 to 10 minutes.
  • Perfect for Baking: Works just like store-bought buttermilk in your favorite recipes.
  • Saves a Trip to the Store: Never worry about running out of buttermilk again.
Buttermilk pie in a pie dish.

Ingredient Notes

See recipe card below this post for ingredient quantities and full instructions.

  • Whole milk: Room-temperature whole milk creates the best texture, but 2% milk will also work.
  • White vinegar or fresh lemon juice: Either acid curdles the milk and gives it the signature tangy flavor.

How to Make Homemade Buttermilk

  1. Measure milk: Measure out 1 cup of whole milk.
  2. Stir: In a small bowl or measuring cup, add 1 tablespoon of white vinegar or fresh lemon juice.
  3. Pour: Pour the white vinegar or lemon juice into the cup of whole milk.
  4. Stir: Stir the mixture gently to combine. You will notice that the milk starts to curdle slightly and thicken. This is what you want to achieve the buttermilk-like consistency.
  5. Rest: Let the homemade buttermilk sit at room temperature for about 5-10 minutes. During this time, the acidity from the vinegar or lemon juice will continue to work on the milk. This is what gives it a tangy flavor and thickens it further.
  6. Use: After resting, your homemade buttermilk is ready to use in your favorite recipes. Try it in pancakes, classic buttermilk biscuits, salad dressings, and more!
Buttermilk waffles topped with butter, maple syrup and fresh mixed berries.

How to Store

  • To Store: Keep homemade buttermilk in an airtight container or jar in the refrigerator for up to 2 weeks. Stir or shake before using.
  • To Freeze: Freeze for up to 3 months in a freezer-safe container. Thaw overnight in the refrigerator and stir well before using.

Frequently Asked Questions

Is it better to use lemon juice or vinegar for buttermilk?

Either option would work just fine. Use what you have at home or in the pantry, and enjoy!

How long will homemade buttermilk last in the fridge?

As long as you keep it covered and stored, buttermilk will stay fresh for up to 2 weeks in the fridge.

Can I freeze buttermilk?

Yes, the texture will be slightly different, but it will still work well in most recipes. Transfer leftover buttermilk to a freezer-safe container, and freeze for up to 3 months. Thaw in the fridge overnight, and stir to combine before using.

Can I use this in any recipe that calls for buttermilk? 

Absolutely! This homemade version can be substituted 1:1 for store-bought buttermilk in baking recipes.

More Helpful Kitchen Tips

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Need help with this recipe?
Click the buttons below to summarize it, adjust for diets, swap ingredients, or ask your own questions!

A liquid measuring cup of homemade buttermilk.

How To Make Homemade Buttermilk

Learn how to make homemade buttermilk in minutes with just milk and lemon juice or vinegar. It works perfectly in all your favorite baking recipes!
5 from 5 votes
Course: Breakfast, Dessert
Cuisine: American
Servings: 1 cup
Prep Time: 5 minutes
Total Time: 5 minutes

Equipment

  • 1 Liquid measuring cup

Ingredients  

  • 1 cup whole milk
  • 1 Tablespoon white vinegar or lemon juice

Instructions 

  • Measure out 1 cup of whole milk. Ensure it's at room temperature for best results.
    1 cup whole milk
  • In a small bowl or measuring cup, add 1 tablespoon of white vinegar or fresh lemon juice. Stir well to combine.
    1 Tablespoon white vinegar
  • Pour the white vinegar or lemon juice into the cup of whole milk.
  • Stir the mixture gently to combine. You will notice that the milk starts to curdle slightly and thicken. This is what you want to achieve the buttermilk-like consistency.
  • Let the homemade buttermilk sit at room temperature for about 5-10 minutes. During this time, the acidity from the vinegar or lemon juice will continue to work on the milk, giving it a tangy flavor and thickening it further.
  • After the resting time, your homemade buttermilk is ready to use in your favorite recipes that call for buttermilk, such as pancakes, biscuits, or salad dressings.

Notes

  • Let the milk come to room temperature before mixing for the best results.
  • Don’t skip the resting time—it allows the milk to thicken properly.
  • Slight curdling is exactly what you want.
  • Measure carefully to maintain the proper ratio of milk to acid.
  • Make only what you need for the freshest flavor.

Nutrition

Serving: 1serving | Calories: 149kcal | Carbohydrates: 11g | Protein: 8g | Fat: 8g | Saturated Fat: 5g | Polyunsaturated Fat: 0.3g | Monounsaturated Fat: 2g | Cholesterol: 29mg | Sodium: 93mg | Potassium: 366mg | Sugar: 12g | Vitamin A: 395IU | Calcium: 301mg
Did you make this recipe?Mention @kasey_allthingsmamma or tag #allthingsmamma!

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About Kasey Schwartz

For over 15 years, Kasey Schwartz, the mastermind behind All Things Mamma, has been the ultimate go-to for mouthwatering family recipes. Her work has been featured on Today.com, Buzz Feed, Better Homes and Gardens, and more, making her your go-to mom next door for all things delicious and family-friendly.

Comments

  1. Toccara Ross says:

    Which makes the best buttermilk the vinegar or the lemon juice?

  2. Plaridel says:

    5 stars
    Make your own homemade buttermilk in minutes with just two simple ingredients! 🥛✨ The easiest kitchen hack you’ll ever learn.

  3. Amelia says:

    5 stars
    So easy — just two ingredients!

  4. Will says:

    5 stars
    Homemade buttermilk saves the day every time.

  5. Lite says:

    5 stars
    Thanks for sharing this— super helpful!

  6. Ellise says:

    5 stars
    I’m so excited to try this!

  7. Judy says:

    Will this work 2% mile?

    1. Shyanne - ATM Team says:

      Yes!

5 from 5 votes

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